Discovery proteomics is a good starting point to evaluate the feasability of a project and to further design an analytical strategy.
It is the method of choice for proteome mapping and identification of PTMs.
We can deploy tailor-made analytical methods that meet the needs of your project. Relative quantification between samples is possible.
Applications that require discovery analysis
• Protein sequence identification
• Proteome mapping
• Label free quantification
• Interactome studies
• Identification of post-translational modifications
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Fast |
Standard |
Deep |
Multidimensional |
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Throughput per sample |
Fast-UHPLC (10 to 30min) |
Standard gradient (90min) |
Long gradient (240 to 300 min) |
Up to 50 h |
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GMass spectrometer |
Q Exactive HF |
Q Exactive plus |
Q Exactive plus |
Q Exactive plus |
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Indicative number of protein IDs* |
700-1500 |
2500 |
5000 |
9000 |
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Applications |
- High throughput screening of large sample cohort |
- Time and cost effective approach |
- Comprehensive study of a limited number of samples/conditions |
- Tailored options on demand |
*based on hela cell lysate
Proteomics platform is part of the Quantitative Biology Unit
Luxembourg Institute of Health
Copyright 2019, LIH, updated in August 2019