NGS (Next-Generation-Sequencing)

Available Library preparation for RNA Sequencing

  • Total RNA-sequencing
  •  mRNA-Sequencing
  • Low Input Total RNA-Sequencing

Please fill the form and send it to luxgen@lih.lu to receive a quote.

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Kit name

 

Company

  

Processing

  

Total RNA Quantity

  

Quality

  

Stranded

TruSeq Stranded Total RNA Library Prep Gold

  

Illumina

  

rRNA and mt-rRNA depletion available for a broad range of species

  

100 ng to 1µg

  

High, lower quality require optimization

  

YES

TruSeq® Stranded mRNA Library Prep

  

Illumina

  

mRNA selection For species with polyA tails

  

0.1 – 1 ug total RNA or 10 - 100 ng previously isolated mRNA (from species with polyA tails)

  

High quality

   YES

Trio RNA-Seq Library Preparation Kit

  

Nugen

  

Depletion to any transcript from any organism

  

500 pg - 50 ng

  

High quality and moderately degraded

   NO

QuantSeq 3´mRNA seq Fwd Library Prep Kit

  

Lexogen

  

Oligo d(T) priming UMI optional Mt-rRNA depletion optional

  

2 ng – 2 µg 500 ng recommended

  

Free of contaminants High quality and moderately degraded

   YES

NEBNext Multiplex Small RNA Library Prep

  

New England Biolabs

  

Gel Size selection

  

125 ng – 1 µg total RNA

  

High Quality RNA

   YES

 

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Library preparation and data analysis are done at LIH. Library Sequencing is done at LNS.